A titrimetric method for the determination of creatine phosphokinase

A titrimetric method for the determination of creating phosphokinase.

A spectrophotometric method for the determination of creatine phosphokinase and myokinase.

where ATP = adenosine triphosphate and ADP= adenosine diphosphate. The substances most commonly determined have been creatine or creatine phosphate; e.g. Banga (1943), Askonas (1951), Narayanaswami (1952), Ennor & Rosenberg (1954), Kuby, Noda & Lardy (1954) and Chappell & Perry (1954). These methods may not always be convenient for the determination of creatine phosphokinase activity in tissue ...

Serum creatine phosphokinase: evaluation of a commercial spectrophotometric method.

The clinical and laboratory applications of an assay method for serum creatine phosphokinase (CPK) utilizing a reagent capsule were investigated. Continuous recording of the change in absorbance at 340 mu, during the assay period gave a slightly sigmoid curve. The time interval between serum addition and the beginning of the linear portion of the absorbancecurve varied with the level of enzyme ...

Cardiac Specific Creatine Phosphokinase

The specificity and sensitivity of serum creatine phosphokinase cardiac specific isoenzyme (MB) in the diagnosis of acute myocardial infarction (AM I) was evaluated. An ion-exchange chromatographic technique was used to isolate MB. Sera layered on mini-columns of DEAE-Sephadex were eluted with Tris-buffered sodium chloride. Quantification of isolated MB was performed by creatine phosphokinase (...

Serum Levels of Creatine Phosphokinase.

Increasing interest in serum enzyme levels in various conditions has led to recognition of the value of the estimation of creatine phosphokinase activity in muscular disorders. Interpretation of the results obtained has been hampered by the lack of adequate information about the ;normal range'. Data for the normal levels of creatine phosphokinase in cord blood, children, and adults are presented.